MOTS-C 100mg Kit

About MOTS-C 100mg Kit

MOTS-c (mitochondrial open reading frame of the 12S rRNA type-c) is a 16-amino-acid peptide encoded not in nuclear DNA but in the mitochondrial genome. It belongs to a small class of mitochondrial-derived peptides, and its discovery established that mitochondria encode signalling molecules acting outside themselves.

This origin is what makes it distinctive: rather than being a synthetic construct or a fragment of a larger nuclear-encoded protein, MOTS-c represents a mitochondrial-to-nuclear signalling channel.

Supplied for laboratory research use only. Not for human consumption.

Research background

Mitochondrial-derived peptide signalling

MOTS-c is translated from a short open reading frame within mitochondrial 12S rRNA. Research has examined how it moves to the nucleus under metabolic stress and influences nuclear gene expression — a retrograde signalling pathway.

AMPK pathway activation

A recurring finding is activation of AMP-activated protein kinase, the cellular energy sensor. AMPK activation is associated with shifts in glucose uptake and fatty-acid oxidation, which forms the basis of the metabolic research interest.

Folate-methionine cycle interaction

Early mechanistic work described interaction with the folate-methionine cycle and consequent effects on purine biosynthesis, linking the peptide to one-carbon metabolism.

Exercise and metabolic models

Animal studies have examined MOTS-c in the context of exercise adaptation and age-related metabolic change, where mitochondrial function is the common thread.

Reconstitution

1. Equilibrate firstAllow the vial to reach room temperature before opening. Introducing solvent into a cold vial promotes condensation, which affects both concentration accuracy and stability.
2. Select the diluentBacteriostatic water — sterile water with 0.9% benzyl alcohol — is standard where the solution will be held for more than a few days. Plain sterile water is appropriate only for single-session use with no storage.
3. Add slowly, down the wallDirect the solvent against the inner wall of the vial rather than onto the powder. A stream striking the lyophilized cake causes localized shear that can denature peptide chains.
4. Do not shakeSwirl gently, or simply let the vial stand. Agitation introduces air and mechanical stress, and both degrade peptides. Full dissolution usually takes a minute or two.
5. Inspect before useA correctly reconstituted solution is clear and colourless. Cloudiness, visible particulate or discolouration indicates a problem with the material or the diluent.

Storage and stability

Lyophilized peptide is stable enough to survive shipping at ambient temperature, which is why cold-chain courier service is not required for transit. Long-term storage is a different matter — material intended to be held for months belongs in a freezer.

Protect from light, and avoid repeated freeze-thaw cycles. Each cycle causes measurable degradation, so dividing a reconstituted stock into single-use aliquots before freezing is preferable to thawing and refreezing one vial repeatedly.

Purity and verification

Every lot is tested by an independent laboratory using two orthogonal methods. HPLC (high-performance liquid chromatography) establishes purity as a percentage of total peptide content, separating the target compound from truncated sequences and synthesis by-products. LC-MS (liquid chromatography–mass spectrometry) confirms molecular identity by mass.

Both matter. Purity alone is insufficient — a 99% pure sample of the wrong compound is still the wrong compound. Together the two tests establish identity and quality, which is why MOTS-c is released only after both pass.

The certificate of analysis references the lot number printed on your vial label. Request the COA for your lot at [email protected].

Published research

Peer-reviewed studies indexed in PubMed. Each links to the abstract and, where a free copy exists, to the full text. We link primary sources rather than summarising them second-hand.

Frequently asked questions

Related compounds

Compounds studied alongside this one, or acting through related pathways.

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